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KMID : 0861020180330050081
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Korea Journal of Herbology
2018 Volume.33 No. 5 p.81 ~ p.88
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Effects of aqueous extract from Taxillus chinensis on monosodium iodoacetate-induced osteoarthritis in rats
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Kim Joong-Sun
Lee A-Young
Moon Byeong-Cheol
Kim Hyo-Seon
Kim Wook-Jin
Kim Chul
Choi Go-Ya
Kim Seung-Hyung
Chun Jin-Mi
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Abstract
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Objectives : The aim of this study was to investigate the protective effects of an aqueous extract from Taxillus chinensis (DC.) Danser (TCE) in Monosodium iodoacetate (MIA)-induced osteoarthritis (OA) rat model.
Methods : Sprague Dawley male rats were divided into the following four groups (n=6 per group): Normal (saline control), MIA (MIA-induced OA with vehicle), TCE (MIA-induced with TCE treatment), and IM (MIA-induced with indomethacin treatment). Rats in which OA was induced by MIA were treated with TCE (200 ㎎/㎏) or indomethacin (1 ㎎/㎏) for 4 weeks. Weight-bearing on the hind legs and body weights were measured weekly. At the end of the experiment (3 weeks after MIA injection), serum aspartate aminotransferase and alanine aminotransferase levels were measured to assess the liver toxicity induced by TCE. Its effects on serum inflammatory cytokine levels and tissue histopathology were also evaluated.
Results : TCE restored the hind limb weight-bearing distribution. Serum levels of Interleukin 6 (IL-6), Tumor necrosis factor alpha (TNF-α) and Leukotriene B4 (LTB4) were significantly higher in the MIA group than in the Normal group, but serum IL-6 levels were significantly lower in the TCE group. In the TCE group, the synovial membrane was protected in hematoxylin and eosin and Safranin-O staining, respectively.
Conclusions : TCE recovered the hind paw weight bearing distribution, inhibited the production of inflammatory cytokine, and protected synovial tissue and cartilage in the OA rat model. Therefore, TCE appears to be an effective therapeutic agent for treating OA and OA-related symptoms.
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KEYWORD
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Taxillus chinensis, osteoarthritis, monosodium iodoacetate, rat, inflammatory cytokine
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